ABSTRACT
Objective:To evaluate the rapid diagnostic value of serum novel coronavirus (2019-nCoV) IgM/IgG detection in COVID-19, aiming to further improve the diagnostic and screening system of COVID-19.Methods:Blood samples were collected from 32 patients with COVID-19 (tested positive for 2019-nCoV nucleic acid by RT-PCR and presented with clinical symptoms) and 34 non-COVID-19 patients (tested negative for 2019-nCoV nucleic acid by RT-PCR and clinically confirmed as non-COVID-19 patients). Colloidal gold-based immunochromatography was used for rapid detection of 2019-nCoV IgM/IgG in these samples. The sensitivity and specificity of the test, and the correlation of serum 2019-nCoV IgM/IgG with disease course were analyzed.Results:Among the 32 COVID-19 patients, nine tested positive for 2019-nCoV IgM with a positive rate of 28.1% (9/32) and 25 positive for 2019-nCoV IgG with a positive rate of 78.1% (25/32). The total positive rate was 84.4% (27/32). Two of the 34 non-COVID-19 patients tested positive for 2019-nCoV IgG with a positive rate of 5.9% (2/34), while none of them was positive for 2019-nCoV IgM. The positive rates of serum IgM were 42.9% (3/7), 30.8% (4/13) and 16.7% (2/12) at 10-20 d, 21-30 d and 31-40 d after the patients developed the symptoms of COVID-19, respectively, which showed a decreasing tread with prolonged disease course. The positive rates of serum IgG in COVID-19 patients were 57.1% (4/7), 84.6% (11/13) and 83.3% (10/12) at 10-20 d, 21-30 d and 30-40 d after symptom onset. The rate showed an increasing trend with prolonged disease course and reached the peak in about 21-30 d.Conclusions:Serum 2019-nCoV IgM/IgG detection (using colloidal gold method) had high sensitivity (84.4%) and strong specificity (94.1%) in the diagnosis of 2019-nCoV infection. It had a great value in the diagnosis and screening of COVID-19 and could be used as a valuable complementary method to the COVID-19 diagnostic system due to its advantages of flexibility, rapidity and simplicity.
ABSTRACT
T A R D N A-binding dom ain protein 43 (T D P-43) is a highly conserved and w idely expressed nuclear protein. N ow adays, the expression of T D P-43 can be found in m ost neurodegenerative diseases such as A lzheim er's disease, w hich m akes it becom e a neurodegenerative disease associated m arker pro-tein. From the current research status at hom eland and abroad, and around the relationship betw een the expression of T D P-43 and brain injury, this article em phatically probes into the specific expression and function of T D P-43 in acute and chronic brain injury based on the know ledge of its biological charac-teristics, w hich aim s to explore the feasibility for determ ining the cause of death and the injury and dis-ability situations by T D P-43 in forensic pathology.
ABSTRACT
Objective To observe the clinical effect of phloroglucinol combined with diazepam in prolonging the latency of primipara and the influence on the safety of mother and infant after medication .Methods A total of 180 cases with a naturally occurring post-partum latency≥8 h and no progress of patients in Weifang Tranditional Chinese Hospital from June 2015 to June 2016 in this study were divided into control group and observation group with 90 cases in each group.The observation group were treated with phloroglucinol combined with diazepam,the control group were treated with diazepam.Two groups appeared uterine inertia when the discretion of intravenous infusion of 0.5% oxytocin injection.Two groups were observed to 16h, still no progression of labor related to obstetric intervention.Observed of cervix big open situation,cervical opened time, cervical edema rate, cervical laceration rate, the rate of vaginal delivery, two hours postpartum hemorrhage, Apgar scores of newborns and the adverse reaction of medicine. Results The duration from drug administration to full dilation of cervix in obseve group was significantly shorter than the control group(P<0.05),the rate of cervical edema in the observation group was significantly lower than the control group(P<0.05), but no statistically difference in the cervical laceration.The the rate of vaginal delivery in observation group decreased significantly when compared with the control group ( P<0.05 ) , two hours postpartum hemorrhage and the Apgar scores of newborns had no significant differcences.Conclusion Puerpera latency prolonged application of phloroglucinol combined with diazepam can significantly speed up the cervix to open the time , and reduce cervical edema and improve vaginal delivery rate,and cervical laceration,two hours postpartum hemorrhage and neonatal complications had no effect.